Deoxyribonucleic Acid Polymerase Associated with Avian Tumor Viruses: Secondary Structure of the Deoxyribonucleic Acid Product
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چکیده
منابع مشابه
Covalent linkage between ribonucleic Acid primer and deoxyribonucleic Acid product of the avian myeloblastosis virus deoxyribonucleic Acid polymerase.
Initiation of deoxyribonucleic acid (DNA) synthesis by the avian myeloblastosis virus DNA polymerase was previously suggested to involve a ribonucleic acid (RNA) primer, the initial product being a DNA molecule joined by a phosphodiester bond to the RNA primer. The existence and nature of such an RNA-DNA joint was investigated by assaying for transfer of a (32)P atom from an alpha-(32)P-deoxyri...
متن کاملSerological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. II. Comparison of avian deoxyribonucleic acid polymerases.
Monospecific antiserum prepared against the isolated deoxyribonucleic acid (DNA) polymerase of avian myeloblastosis virus (AMV) neutralized the endogenous ribonucleic acid-instructed DNA polymerase activity of detergent-disrupted virus. The viral polymerase was serologically unrelated to the seven major structural polypeptides of AMV. Furthermore, the viral enzyme was distinguished from normal ...
متن کاملDeoxyribonucleic Acid Polymerase III of Escherichia coli
DNA polymerase III has been purified 4,500-fold from the Escherichia coli mutant, HMS83, which lacks DNA polymerases I and II. When subjected to disc gel electrophoresis, the most purified fraction exhibits a single major protein band from which enzymatic activity may be recovered. Polyacrylamide gel electrophoresis under denaturing conditions produces two protein bands with molecular weights o...
متن کاملParticipation of deoxyribonucleic acid polymerase alpha in amplification of ribosomal deoxyribonucleic acid in Xenopus laevis.
Aphidicolin, a known inhibitor of eucaryotic deoxyribonucleic acid (DNA) polymerase alpha, efficiently inhibited amplification of ribosomal DNA during oogenesis in Xenopus laevis. DNA polymerase alpha, but not DNA polymerase gamma, as isolated from ovaries, was sensitive to aphidicolin. DNA polymerase beta was not detectable in Xenopus ovary extracts. Therefore, DNA polymerase alpha plays a maj...
متن کاملRole of deoxyribonucleic acid polymerase beta in nuclear deoxyribonucleic acid synthesis.
Exonuclease activity has been measured in preparations of both enzymes. The activity associated with DNA polymerase a requires denatured DNA, Mg2+ and is ATP-dependent. The polymerase b-associated nuclease requires denatured DNA and Mg2+ or Caz+. Studies of the distribution of these enzymes during the cell cycle have shown that extracts of cells harvested at the time of nuclear DNA synthesis (C...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1971
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.7.1.77-86.1971